Introduction

Overview

Teaching: 5 min
Exercises: 0 min
Questions
• What sort of scientific questions can we answer with image processing / computer vision?

• What are morphometric problems?

• What are colorimetric problems?

Objectives
• Recognize scientific questions that could be solved with image processing / computer vision.

• Recognize morphometric problems (those dealing with the number, size, or shape of the objects in an image).

• Recognize colorimetric problems (those dealing with the analysis of the color or the objects in an image).

We can use relatively simple image processing and computer vision techniques in Python, using the OpenCV library. With careful experimental design, a digital camera or a flatbed scanner, in conjunction with some Python code, can be a powerful instrument in answering many different kinds of problems. Consider the following two types of problems that might be of interest to a scientist.

Morphometrics

Morphometrics involves counting the number of objects in an image, analyzing the size of the objects, or analyzing the shape of the objects. For example, we might be interested automatically counting the number of bacterial colonies growing in a Petri dish, as shown in this image: We could use image processing to find the colonies, count them, and then highlight their locations on the original image, resulting in an image like this: Colorimetrics

Colorimetrics involves analyzing the color of objects in an image. For example, consider this video of a titrant being added to an analyte (click on the image to see the video): We could use image processing to look at the color of the solution, and determine when the titration is complete. This graph shows how the three component colors (red, green, and blue) of the solution change over time; the change in the solution’s color is obvious. Why write a program to do that?

Note that you can easily manually count the number of bacteria colonies shown in the morphometric example above. Why should we learn how to write a Python program to do a task we could easily perform with our own eyes? There are at least two reasons to learn how to perform tasks like these with Python and OpenCV:

1. What if there are many more bacteria colonies in the Petri dish? For example, suppose the image looked like this: Manually counting the colonies in that image would present more of a challenge. A Python program using OpenCV could count the number of colonies more accurately, and much more quickly, than a human could.

2. What if you have hundreds, or thousands, of images to consider? Imagine having to manually count colonies on several thousand images like those above. A Python program using OpenCV could move through all of the images in seconds; how long would a graduate student require to do the task? Which process would be more accurate and repeatable?

As you can see, the simple image processing / computer vision techniques you will learn during this workshop can be very valuable tools for scientific research.

As we move through this workshop, we will return to these sample problems several times, and you will solve each of these problems during the end-of-workshop challenges.

Let’s get started, by learning some basics about how images are represented and stored digitally.

Key Points

• Simple Python and OpenCV techniques can be used to solve genuine morphometric and colorimetric problems.

• Morphometric problems involve the number, shape, and / or size of the objects in an image.

• Colorimetric problems involve analyzing the color of the objects in an image.